Scientific Program

Conference Programm with abstracts can be downloaded here

Monday, October 3

13:00-19:00	RUBIN HALL	REGISTRATION
14:00-14:20	RUBIN HALL	CONFERENCE OPENING (Alexander Savitsky, Wolfgang Becker) „Activities of the DWIH in Moscow. Tools for funding and intensification of German-Russian cooperation“ Anna Savostina, German House for Research and Innovation (DWIH) in Moscow,
14:20-16:40	RUBIN HALL	FlUORESCENCE AND PHOSPHORESCENCE LIFETIME IMAGING (Chairmen: Ammasi Periasamy, Elena Zagaynova)
14:20		Wolfgang Becker Becker&Hickl GmbH, Berlin, Germany Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC: Advanced Techniques and Applications
14:50		Michael Roberts (invited) University of Queensland, Brisbane, Australia Using multiphoton tomography with fluorescence lifetime imaging to characterize tissue morphology and exogenous material transport in organs
15:20		Klaus Suhling (invited) Kings College, London, UK Wide-field Time-Correlated Single Photon Counting FLIM
15:50		Elena Zagaynova (invited) Nizhny Novgorod State Medical Academy, Nizhniy Novgorod, Russia Tumor metabolism: fluorescence imaging with autofluorophors and genetically encoded sensors
16:20-16:40		COFFEE BREAK
16:40-19:50	RUBIN HALL	FlUORESCENCE AND PHOSPHORESCENCE LIFETIME IMAGING (continuation)
16:40		Dusan Chorvat (invited) International Laser Center, Bratislava, Slovakia Advanced imaging and spectroscopy of intrinsic fluorophores
17:10		Marina Shirmanova Nizhniy Novgorod State Medical Academy, Nizhniy Novgorod, Russia Measuring viscosity in cancer using molecular rotors and FLIM
17:30		Sviatlana Kalinina Ulm University, Ulm, Germany Simultaneous NADH-FLIM and oxygen sensing PLIM for metabolic mapping
17:45		Thomas Gensch (invited) Research Centre Jülich, Germany Intracellular ion concentration in living cells and tissue determined by FLIM
18:15		Michael Börsch (invited) Jena University, Jena, Germany Observing the rotary motors of F oF1 -ATP synthase at work using single-molecule FRET
18:45		Ilya Turchin (invited) Institute of Applied Physics of the RAS, Nizhniy Novgorod, Russia Fluorescent small-animal imaging with genetically encoded sensors
19.10		Patrick Schaefer Ulm University, Ulm, Germany Imaging Mitochondrial Function in Alzheimer´s Disease
19:30		Anastasia Belova Institute of Applied Physics of the RAS, Nizhniy Novgorod, Russia Fluorescence sensing of hydrogen peroxide level changes under cisplatin treatment of tumor cells

Tuesday, October 4

8:30-11:05	CONGRESS HALL	DIFFUSE OPTICAL IMAGING AND CLINICAL IMAGING Chairmen: Valery Tuchin, Heidrun Wabnitz
8:30		Valery Tuchin (invited) Saratov National Research State University, Saratov, Russia Tissue and cell optical clearing as a tool for enhanced microscopy and imaging: from in vitro to in vivo
9:00		Heidrun Wabnitz (invited) PTB Berlin, Germany In-vivo time-domain diffuse optical imaging of the adult human brain
9:30		Karsten Koenig (invited) University Saarbruecken, Saarbruecken, Germany Multiphoton tomography of astronauts
9:50		Konovalov Alexander Russian Federal Nuclear Center – Zababakhin Institute of Applied Physics, Snezhinsk, Russia An analytic perturbation model for high-resolution time-domain diffuse optical tomography in the flat layer transmission geometry
10:10		Yoko Miura (invited) University Lübeck, Lübeck, Germany Fluorescence lifetime in retinal cell pathology
10:30		Martin Hammer (invited) University Jena, Jena, Germany Pathologic alterations in clinical FLIM at the ocular fundus – lessons learned from two photon FLIM microscopy in vitro
10:50		Innesa Ferulova Institute of Atomic Physics and Spectroscopy, University of Latvia, Riga, Latvia Correlation of skin autofluorescence photobleaching rate and the lifetime component
11:05-11:30		COFFEE BREAK
11:30-13:30	CONGRESS HALL	SUPER RESOLUTION MICROSCOP AND SINGLE MOLECULAR DETECTION Chairmen: Jerker Widengren, Marcel Leutennegger
11:30		Jerker Widengren (invited) KTH, Royal Institute of Technology, Stockholm, Sweden Fluorescence fluctuation and super-resolution techniques - fundamental biomolecular studies and towards clinical diagnostics
12:00		Ago Rinken (invited) University of Tartu, Insitute of Chemistry, Tartu, Estonia Fuorescence-based methods for monitoring lgand binding kinetics to GPCR
12:30		Vladislav Shcheslavskiy Becker & Hickl GmbH, Berlin, Germany Scanning near-field optical microscopy enhanced with fluorescence lifetime imaging
12:50		Herman Fennema Nikon Instruments Europe B.V., Netherlands Advanced Super Resolution Microscopy Technologies from Nikon
13:10		Natalia Klementieva Nizhniy Novgorod State Medical Academy, Nizhniy Novgorod, Russia Fine structure of actin cytoskeleton in cancer cells and tissues unraveled by fluorescence imaging
13:30-14:30		LUNCH
14:30-16:00	CONGRESS HALL	ADFLIM PLENAR Moderators: Vladislav Shcheslavskiy, Alexander Savitsky
14:30		Enrico Gratton (invited) University of California,-Irvine, USA Metabolic changes in cells and tissues revealed by FLIM of intrinsic autofluorescence
15:15		Anna Moore (invited) Massachusetts General Hospital, Harvard Medical School, Boston, USA Image-guided Precision Nanomedicine for Cancer Therapy
17:00-17:30	CONGRESS HALL	FORUM OPENING Chairmen: Anatoliy Grigoriev, Yuriy Natochin, Revaz Sepiashvili, Vadim Ivanov, Alexander Gabibov, Alexander Savitsky, Arieh Warshel, Christopher Contag, Vsevolod Tkachuk, Michail Ostrovskiy, Valeriy Chereshnev, Alain Krol, Michael Blackburn
17:30-19:40	CONGRESS HALL	FORUM PLENAR
17:30		Arieh Warshel University of Southern California, Los Angeles, USA Advancing of computer modeling of biochemical processes at molecular level
18:15		Vsevolod Tkachuk Lomonosov Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia V.P. Demihov –outstanding Russian surgeon, transplantologist, physiologist
19:00		Christopher Contag Stanford University, USA, President of the World Molecular Imaging Society (WMIS) Imaging Biology in Living Animals and Humans
20:00-21:30		WELCOME COCKTAIL

Wednesday, October 5

8:30-10:45	RUBIN HALL	PROBE CHEMISTRY Chairmen: Alexey Bogdanov, Dmitriy Papkovskiy
8:30		Alexander Savitsky (invited) FRC of Biotechnology, Moscow, Russia SAASOTI as a probe for the super resolution microscopy
9:00		Konstantin Lukyanov (invited) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia Towards high-photostability imaging of live cells
9:30		Marcel Leutenegger (invited) Max Planck Institute for Biophysical Chemistry, Gottingen, Germany Synthetic fluorophores for GSDIM: screening and image analysis
10:00		Alexey Bogdanov (invited) Department of Radiology University of Massachusetts Medical School, Boston, USA Probes and sensors for near-infrared imaging of enzymatic activity and protein-DNA interactions
10:30		Dmitry Gorbachev Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia Green fluorescent proteins with long fluorescence lifetime
11:15-11:30		COFFEE BREAK
10:45-15:05	CONGRESS HALL	FORUM PLENAR (Forum program http://www.rusbiochem.org/page99.html)
12:40-13:25	RUBIN HALL	RUSSIAN MOLECULAR IMAGING SOCIETY FOUNDING CONFERENCE
13:30-14:20		LUNCH
14:20-15:40	RUBIN HALL	PROBE CHEMISTRY (continuation)
14:20		Wolfgang Becker (invited) Becker&Hickl GmbH, Berlin, Germany Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation
14:50		Dmitriy Papkovskiy (invited) University of Cork, Cork, Ireland Imaging of oxygenation and cellular function in 3D tissue models by multiplexed PLIM/FLIM
15:10		Ugarova Natalia (invited) Lomonosov Moscow State University, Moscow, Russia Firefly luciferase as a probe for imaging and monitoring in living systems
16:20-18:30	RUBIN HALL	NEUROSCIENCE Chairmen: Alexey Semyanov, Konstantin Lukyanov
16:20-16:50		Alexey Semyanov Institute of Neuroscience University of Nizhniy Novgorod Cellular and subcellular optical imaging in neuroscience
16:50-17:20		Vsevolod Belousov Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia Thermogenetic stimulation of neurons with single-cell resolution
17:20-17:50		Ilya Fedotov Lomonosov Moscow State University, Russia Fiber-optic neurointerfaces for fluorescence brain imaging
17:50-18:10		Olga Ivashkina Department of Neuroscience, NBICS-center, NRC "Kurchatov Institute", Moscow, Russia Neuronal encoding of conditioned signals in the mouse parietal cortex: in vivo two-photon imaging
18:10-18:30		Franco Klingberg Thermo Fisher Scientific, Darmstadt, Germany New Technologies and Reagents for Live Cell Imaging
8:30-13:00	RUBIN HALL	POSTER SESSION Moderators: Alexander Savitsky, Wolfgang Becker
		1. Arseny Aybush Semenov Institute of Chemical Physics of the RAS, Moscow, Russia Biological objects visualization in chirped CARS microscopy
		2. Ekaterina Boruleva FRC of Biotechnology of the RAS ,Moscow, Russia The study of endogenous fluorescence of living cells of mammals by FLIM
		3. Varvara Dudenkova Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia Quantitative characteristic collagen changes by SHG signal on different biological models
		4. Nadezhda Gurskaya Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia Fluorescence labeling of proteins in live cells using heterodimerization of artificial coiled coils
		5. Natalia Kazachkina Federal Research Centre “Fundamentals of biotechnology”, A.N. Bach Institute of biochemistry, Russian Academy of science, Russia Study of fluorescence properties of caspase-3 sensor in tumor cells under the influence of antitumor agents
		6. Sergei Kopanchuk Institute of Chemistry, University of Tartu, Tartu, Estonia Allosteric modulation of peptide ligand binding to Neuropeptide Y receptor Y1 revealed by integrative fluorescence data Global Analyses
		7. Anton Radaev, Elena Koshel St-Petersburg State University, Russia A new lipophilic phosphorescent probe used for two-photon bioimaging
		8. Elena Koshel St-Petersburg State University, Russia New phosphorescent probes based on transition metals complexes for Phosphorescent Lifetime Imaging Microscopy (PLIM)
		9. Alexander Konovalov, Alexander Uglov Russian Federal Nuclear Center – Zababakhin Institute of Applied Physics, Snezhinsk; Russia An analytic perturbation model for high-resolution time-domain diffuse optical tomography in the flat layer transmission geometry
		10. Rufina Mardanova Moscow Technological University, Moscow, Russia Caspase sensor visualization in 3D cancer cell models
		11. Nadezhda Marynich Lomonosov Moscow State University, Moscow, Russia Isolation, purification and characterization of properties of caspase-3 sensors ТR-M5-К и ТR-M6-К
		12. Michael Samtsov Sevchenko Research Institute of Applied Phisical Problems, Minsk, Belarus The fluorescent features of indotricarbocyanine dyes in biotissues
		13. Leonid Shaposhnikov Lomonosov Moscow State University, Moscow, Russia The studying of oligomeric state of caspase-3 FRET-sensors TR-M5-K and TR-M6-K
		14. Ilya Solovyev FRC of Biotechnology of the RAS ,Moscow, Russia New fast photoconvertible protein SAASoti
		15.Alexei Vedyaykin Research Institute of Nanobiotechnologies, Peter the Great St.Petersburg Polytechnic University, Saint-Petersburg, Russia Super-resolution fluorescence microscopy for investigation of bacterial cytoskeleton

Thursday, October 6

8:30-11:20

RUBIN HALL

FLIM-FRET Chairmen: Enrico Gratton, Claus Seidel

8:30

Yves Mely (invited) University of Strasbourg, Strasbourg, France
Quantitative and high resolution fluorescence imaging techniques for investigating intracellular interactions and dynamics of HIV-1 proteins

9:00

Ammasi Periasamy (invited) University of Virginia, Charlottesville, USA
Investigation of prostate cancer in live specimens using FLIM-FRET microscopy

9:30

Claus Seidel (invited) University of Düsseldorf, Düsseldorf, Germany
Watching structure and dynamics of proteins and protein complexes by high-precision FRET in vitro and in live cells

10:00

Piotr Wardega NanoTemper Technologies GmbH, Krakow, Poland
Advanced quantitative biomolecular analytics in free solution

10:30

Raul Bukowiecki Max Delbrueck Center for Molecular Medicine, Berlin, Germany; Free University, Berlin, German
Imaging Detection of protein misfolding in Huntington’s disease model systems with sensitive TR-FRET-based lifetime imaging

10:45

Alexander Goryashchenko FRC of Biotechnology of the RAS , Moscow, Russia
Rationally designed peptide molecular beacon for highly efficient FRET-sensors

11:00

Victoria Zherdeva FRC of Biotechnology of the RAS, Moscow, Russia
FLIM-FRET of genetically encoded sensor of caspase 3 in tumor xenografts

11:15

Darya Smirnova Lomonosov Moscow State University, Moscow, Russia
Bioluminescence Resonance Energy Transfer system based on Firefly L. mingrelica Luciferase and its application for the Rapid Homogeneous Immunoassay

11:30-12:00

COFFEE BREAK

10:45-16:05

CONGRESS HALL

FORUM PLENAR (Forum program http://www.rusbiochem.org/page99.html)

13:30-14:30

LUNCH

16:15-17:20

RUBIN HALL

MODERN FLUORESCENT MICROSCOPY Chairmen: Karsten Koenig, Ago Rinken

16:15

Tatiana Sergeeva Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia
Analysis of intracellular pH and metabolic changes in cancer cells during apoptosis using FLIM-FRET imaging

16:30

Natalia Morozova Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia
Study of restriction-modification system Esp1396I in E. coli at the single-cell level using fluorescence microscopy

16:50

Andrey Bogorodskiy Moscow Institute of Physics and Technology, Moscow, Russia
Nucleation and growth of membrane protein crystals in meso – a fluorescence microscopy

17:05

Natalia Rovnyagina M.V. Lomonosov Moscow State University, Department of Physics, Moscow, Russia Different stages of fibril formation as seen in intrinsic and extrinsic fluorescence tudy

17:10-17:30

RUBIN HALL

CLOSING OF THE ADFLIM CONFERENCE